97久久久精品综合88久久_亚洲国产精品一_久热热国产久热_97操操操_北条麻妃在线免费观看_精品国自产拍天天拍

掃碼關(guān)注公眾號           掃碼咨詢技術(shù)支持           掃碼咨詢技術(shù)服務(wù)
  
客服熱線:400-901-9800  客服QQ:4009019800  技術(shù)答疑  技術(shù)支持  質(zhì)量反饋  關(guān)于我們  聯(lián)系我們
аⅴ天堂最新版在线中文,在线视频免费黄,亚洲.国产.中文慕字在线
首頁 > 產(chǎn)品中心 > 一抗 > 產(chǎn)品信息
phospho-SIRT1 (Thr530) Recombinant Rabbit mAb (bsm-52202R)  
訂購熱線:400-901-9800
訂購郵箱:sales@bioss.com.cn
訂購QQ:  400-901-9800
技術(shù)支持:techsupport@bioss.com.cn
50ul/1400.00元
100ul/2500.00元
大包裝/詢價

產(chǎn)品編號 bsm-52202R
英文名稱 phospho-SIRT1 (Thr530) Recombinant Rabbit mAb
中文名稱 磷酸化沉默調(diào)節(jié)蛋白1重組兔單抗
別    名 SIR2L1; NAD-dependent protein deacetylase sirtuin-1; EC:2.3.1.286; hSIRT1; NAD-dependent protein deacylase sirtuin-1; Regulatory protein SIR2 homolog 1; SIR2-like protein 1(hSIR2); SirtT1 75 kDa fragment; SIR1_HUMAN;  
產(chǎn)品類型 磷酸化抗體 重組兔單抗 
研究領(lǐng)域 細(xì)胞生物  細(xì)胞凋亡  表觀遺傳學(xué)  
抗體來源 Rabbit
克隆類型 Recombinant
克 隆 號 6H5
交叉反應(yīng) Human
產(chǎn)品應(yīng)用 WB=1:500-1000,IHC-P=1:100-500,IHC-F=1:50-200,IF=1:50-200,ICC/IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 81 kDa
檢測分子量
細(xì)胞定位 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthesised phosphopeptide derived from human SIRT1 around the phosphorylation site of Thr530: PP(p-T)PL 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]

Subcellular Location:
Cytoplasm. Mitochondrion and Nucleus > PML body. Cytoplasm. Recruited to the nuclear bodies via its interaction with PML. Colocalized with APEX1 in the nucleus. May be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane. Shuttles between nucleus and cytoplasm.

Tissue Specificity:
Widely expressed.

Post-translational modifications:
Methylated on multiple lysine residues; methylation is enhanced after DNA damage and is dispensable for deacetylase activity toward p53/TP53.
Phosphorylated. Phosphorylated by STK4/MST1, resulting in inhibition of SIRT1-mediated p53/TP53 deacetylation. Phosphorylation by MAPK8/JNK1 at Ser-27, Ser-47, and Thr-530 leads to increased nuclear localization and enzymatic activity. Phosphorylation at Thr-530 by DYRK1A and DYRK3 activates deacetylase activity and promotes cell survival. Phosphorylation by mammalian target of rapamycin complex 1 (mTORC1) at Ser-47 inhibits deacetylation activity. Phosphorylated by CaMK2, leading to increased p53/TP53 and NF-kappa-B p65/RELA deacetylation activity (By similarity). Phosphorylation at Ser-27 implicating MAPK9 is linked to protein stability. There is some ambiguity for some phosphosites: Ser-159/Ser-162 and Thr-544/Ser-545.
Proteolytically cleaved by cathepsin B upon TNF-alpha treatment to yield catalytic inactive but stable SirtT1 75 kDa fragment (75SirT1).
S-nitrosylated by GAPDH, leading to inhibit the NAD-dependent protein deacetylase activity.

Similarity:
Belongs to the sirtuin family. Class I subfamily.
Contains 1 deacetylase sirtuin-type domain.

SWISS:
Q96EB6

Gene ID:
23411

Database links:

Entrez Gene: 23411 Human

Entrez Gene: 93759 Mouse

Entrez Gene: 309757 Rat

Omim: 604479 Human

SwissProt: Q96EB6 Human

SwissProt: Q923E4 Mouse

Unigene: 369779 Human

Unigene: 351459 Mouse



產(chǎn)品圖片
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Phospho-SIRT1(T530) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (bsm-52717R7, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC staining of Phospho-SIRT1(T530) in 293 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (bsm-52717R7, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor?488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of Phospho-SIRT1(T530) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (bsm-52717R7, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor?488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of Phospho-SIRT1(T530) in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (bsm-52717R7, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor?488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
版權(quán)所有 2004-2026 www.kastlife.cn 北京博奧森生物技術(shù)有限公司
通過國際質(zhì)量管理體系ISO 9001:2015 GB/T 19001-2016    證書編號: 00124Q34771R2M/1100
通過國際醫(yī)療器械-質(zhì)量管理體系ISO 13485:2016 GB/T 42061-2022    證書編號: CQC24QY10047R0M/1100
京ICP備05066980號-1         京公網(wǎng)安備110107000727號
主站蜘蛛池模板: 国产一区色 | 欧美精产国品一二三产品区别在哪 | 我和亲妺妺乱的性视频 | 51成人| 日本黄色视 | 日本一区二区三区精品福利视频 | 337P亚洲日本中国大胆69 | 制服丝袜中文字幕无码自拍 | 欧美日本在线一区 | 欧美日韩国产va另类 | 青青草华人在线 | 最近免费中文字幕中文高清 | 国产精品久久久久9999鸭 | 久久不卡视频 | 麻豆乐园| 成人春色视频www | 成网在线看 | 成人毛片一级 | 亚洲视频区 | 国产精品偷伦在线观看 | 国产初高中生露脸在线播放 | 精品国产一区天天 | 日韩一区二区在线观看视频 | 488成人啪啪片 | 欧美一区a| 国产亚洲AV夜间福利香蕉149 | 久久WWW成人看片免费不卡 | 日韩av成人 | 欧美亚洲欧美 | 精品男人天堂 | 亚洲国产成人精品无码区在线观看 | 91精品视频网址 | 一区二区三区四区影院 | 国内精品国产三级国产a久久 | 蜜桃视频成人m3u8 | 亚洲日韩欧美内射教官 | 色窝窝无码精品一区二区 | 国产亚洲精品第一综合不卡 | a级大片免费观看 | 欧洲熟妇乱xxxxx大屁股7 | 日韩欧美中文字幕在线四区 |