| 產品編號 | bs-48004P |
| 英文名稱 | Recombinant human TNF-Alpha protein (Active, HEK293) |
| 中文名稱 | 重組人腫瘤壞死因子α (活性的, Tag free) |
| 別 名 | Tumor necrosis factor-α; APC1; Cachectin; DIF; Differentiation inducing factor; Macrophage cytotoxic factor; MCF; Necrosin; TNF a ; TNF alpha; TNFalpha; TNF; TNF Macrophage Derived; TNF Monocyte Derived; TNF Superfamily Member 2; TNFA; TNFSF2; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor Precurso; Tumour Necrosis Factor Alpha. ;Recombinant human TNF-Alpha protein (Active, HEK293) 腫瘤壞死因子α重組蛋白; 腫瘤壞死因子α; 重組人腫瘤壞死因子α; 重組人TNF α; |
| 克 隆 號 | |
| 理論分子量 | 17 kDa |
| 檢測分子量 | 17 |
| 性 狀 | Lyophilized |
| 濃 度 | ＞0.5 mg/ml |
| 物 種 | Human |
| 序 列 | 77-233/233 |
| 純 度 | >95% as determined by SDS-PAGE |
| 純化方法 | AC |
| 內毒素 | <0.5 EU/μg as determined by LAL. |
| 表達系統 | HEK293 cell |
| 活性 | Yes |
| 標簽 | Tag free |
| 緩 沖 液 | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| 保存條件 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after r |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品介紹 |
Tumor necrosis factor alpha (TNFα) is an EGF related polypeptide growth factor that signals through the TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and also produced by monocytes, keratinocytes, and various tumor cells. TNFα stimulates the proliferation of a wide range of epidermal and epithelial cells. TNFα is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia. Under certain conditions it can stimulate cell proliferation and induce cell differentiation, impair regulatory T cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. This recombinant human TNF alpha (Glycosylated homotrimer) is produced by human cells.(Tag free) Biological activity: The activity was measured by the cytotoxicity in L-929 cell in the presence of actinomycin D. Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin. |
| 產品圖片 |
The purity of the protein is greater than 95% as determined by non reduced and reduced SDS-PAGE.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
