| 產品編號 | bs-48006P |
| 英文名稱 | Recombinant human IL-12 protein (Active, HEK293) |
| 中文名稱 | 重組人白介素12 (活性的, Tag free) |
| 別 名 | IL12; IL-12; CLMF p35; CLMF1; CTL maturation factor (TcMF); Cytotoxic lymphocyte maturation factor 1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL 12 subunit p35; IL12A; Interleukin 12 alpha chain; Interleukin 12 p35; Interleukin 12A; Natural killer cell stimulatory factor 1; NK cell stimulatory factor chain 1; NKSF1; p35; IL12A_HUMAN. ;Recombinant human IL-12 protein (Active, HEK293) 人白介素12 重組蛋白; 人白細胞介素12 ; 重組人白介素12; 重組人IL-12; |
| 克 隆 號 | |
| 理論分子量 | 57 kDa |
| 檢測分子量 | 60 |
| 性 狀 | Lyophilized |
| 濃 度 | ＞0.5 mg/ml |
| 物 種 | Human |
| 序 列 | 23-219/219 |
| 純 度 | >95% as determined by SDS-PAGE |
| 純化方法 | AC |
| 內毒素 | <0.5 EU/μg as determined by LAL. |
| 表達系統 | HEK293 cell |
| 活性 | Yes |
| 標簽 | Tag free |
| 緩 沖 液 | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| 保存條件 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after r |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品介紹 |
IL-12 is a disulfide-linked heterodimeric protein (p70), composed of two subunits, p35 and p40, which are encoded by two different genes. IL-12 is predominantly produced by macrophages and B lymphocytes. IL-12 functions as a growth factor for activated T and NK cells, enhences the lytic activity of NK/lymphokine activated killer cells, and stimulates the production of IFN-γ by resting PBMC. This recombinant human IL-12 is produced by human cells.(Tag free) Biological activity: The activity was measured by its ability to stimulate the proliferation of PHA transformed human peripheral blood lymphoblast. Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin. |
| 產品圖片 |
The purity of the protein is greater than 95% as determined by non reduced and reduced SDS-PAGE.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
