| 產品編號 | bsm-60233R |
| 英文名稱 | CD22 Recombinant Rabbit mAb |
| 中文名稱 | B淋巴細胞粘附分子CD22重組兔單抗 |
| 別 名 | B-cell receptor CD22; B cell receptor CD22 precursor; B lymphocyte cell adhesion molecule; BL CAM; BL-CAM; BLCAM; CD 22; CD22 antigen; CD22 molecule; CD22 protein; Leu14; Lyb8; MGC130020; Sialic acid binding immunoglobulin like lectin 2; SIGLEC 2; SIGLEC2; SIGLEC-2; FLJ22814; MGC130020; CD22_HUMAN; B-lymphocyte cell adhesion molecule; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14. |
| 研究領域 | 腫瘤 免疫學 干細胞 細胞粘附分子 細胞表面分子 細胞類型標志物 淋巴細胞 b-淋巴細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | G12H9 |
| 交叉反應 | Human |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 91 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | A synthesized peptide derived from human CD22: 1-59/847 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Predicted to enable CD4 receptor binding activity; protein phosphatase binding activity; and sialic acid binding activity. Involved in B cell activation; negative regulation of B cell receptor signaling pathway; and regulation of endocytosis. Located in early endosome and recycling endosome. [provided by Alliance of Genome Resources, Nov 2021] SWISS: P20273 Gene ID: 933 Database links: Entrez Gene: 933 Human SwissProt: P20273 Human |
| 產品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with CD22 monoclonal antibody, unconjugated (bsm-60233R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD22 Monoclonal Antibody, Unconjugated(bsm-60233R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD22 Monoclonal Antibody, Unconjugated(bsm-60233R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
