| 產(chǎn)品編號(hào) | bsm-60240R |
| 英文名稱 | CD79b Recombinant Rabbit mAb |
| 中文名稱 | CD79b重組兔單抗 |
| 別 名 | AGM6; B cell antigen receptor complex associated protein beta chain; B cell specific glycoprotein B29; B-cell antigen receptor complex-associated protein beta chain; B-cell-specific glycoprotein B29; B29; B29/Ig-beta/CD79b; CD 79b; CD79b antigen; CD79b molecule; CD79b molecule immunoglobulin associated beta; CD79b protein; CD79B_HUMAN; Ig beta; Ig-beta; IGB; Igbeta; Immunoglobulin associated B29; Immunoglobulin associated B29 protein; Immunoglobulin associated beta; Immunoglobulin associated protein; Immunoglobulin-associated B29 protein; MGC108607. |
| 研究領(lǐng)域 | 免疫學(xué) b-淋巴細(xì)胞 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | C7H9 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1:20-50,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 23 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | A synthesized peptide derived from human CD79b: 3-54 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-beta protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] SWISS: P40259 Gene ID: 974 Database links: Entrez Gene: 974 Human Entrez Gene: 15985 Mouse SwissProt: P40259 Human SwissProt: P15530 Mouse |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with CD79b monoclonal antibody, unconjugated (bsm-60240R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD79b Monoclonal Antibody, Unconjugated(bsm-60240R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD79b Monoclonal Antibody, Unconjugated(bsm-60240R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
4% Paraformaldehyde-fixed Ramos (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (CD79b) monoclonal Antibody, unconjugated (bsm-60240R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
(UPPER PANEL) 4% Paraformaldehyde-fixed Ramos (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (CD79b) monoclonal Antibody, unconjugated (bsm-60240R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
(LOWER PANEL) MCF-7/Knockout Sample (H) cell as Negative Control. The experimental procedure was as same as above.
The Ramos(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-CD79b antibody (bsm-60240R): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-60295G-FITC): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
|
