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IKZF1 Recombinant Rabbit mAb (bsm-60278R)  
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產品編號 bsm-60278R
英文名稱 IKZF1 Recombinant Rabbit mAb
中文名稱 IKZF1重組兔單抗
別    名 IKAROS family zinc finger 1(Ikaros); PRO0758; CLL associated antigen KW 6; DNA-binding protein Ikaros; hIk 1; hIk-1; IK1; Ikaros(zinc finger protein); IKAROS; Ikaros family zinc finger protein 1; IKZF1_HUMAN; LYF1; Lymphoid transcription factor LyF-1; zinc finger protein subfamily 1A 1; ZNFN1A1.  
研究領域 腫瘤  轉錄調節因子  
抗體來源 Rabbit
克隆類型 Recombinant
克 隆 號 IK3C9
交叉反應 Human
產品應用 WB=1:500-2000,IHC-P=1:1000,IHC-F=1:50-100,IF=1:50-100,ICC/IF=1:50
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 57 kDa
檢測分子量
細胞定位 細胞核 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human IKZF1 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors. Overexpression of some dominant-negative isoforms have been associated with B-cell malignancies, such as acute lymphoblastic leukemia (ALL). [provided by RefSeq, May 2014]

SWISS:
Q13422

Gene ID:
10320

Database links:

Entrez Gene: 10320 Human

SwissProt: Q13422 Human



產品圖片
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Lysate: Raji, Ramos, MOLT4, Jurkat Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 58 kDa Observed MW: 50-70 kDa
Tissue:Human lung adenocarcinoma Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60278R
Tissue:Human colon cancer Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60278R
Tissue:Human lymphoma Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60278R
Cell line: Jurkat Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for bsm-60278R
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