| 產品編號 | bsm-54396R |
| 英文名稱 | ADH1A Recombinant Rabbit mAb |
| 中文名稱 | 乙醇脫氫酶1重組兔單抗 |
| 別 名 | ADH1A_HUMAN; EC:1.1.1.1; Alcohol dehydrogenase 1A; ADH1; Alcohol dehydrogenase subunit alpha; |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 14D1 |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500,ICC/IF=1:50-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 41 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human ADH1A |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a member of the alcohol dehydrogenase family. The encoded protein is the alpha subunit of class I alcohol dehydrogenase, which consists of several homo- and heterodimers of alpha, beta and gamma subunits. Alcohol dehydrogenases catalyze the oxidation of alcohols to aldehydes. This gene is active in the liver in early fetal life but only weakly active in adult liver. This gene is found in a cluster with six additional alcohol dehydrogenase genes, including those encoding the beta and gamma subunits, on the long arm of chromosome 4. Mutations in this gene may contribute to variation in certain personality traits and substance dependence. [provided by RefSeq, Nov 2010] Subcellular Location: Cytoplasmic. Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. SWISS: P07327 Gene ID: 124 Database links: Entrez Gene: 124 Human Entrez Gene: 11522 Mouse SwissProt: P07327 Human SwissProt: P00329 Mouse |
| 產品圖片 |
Sample:
Lane 1: Rat Liver tissue lysates
Primary: Anti-ADH1A (bsm-54396R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.
ICC staining Alcohol Dehydrogenase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
