| 產(chǎn)品編號(hào) | bsm-60064M |
| 英文名稱 | Acetyl-Histone H4 (Lys16) Recombinant Mouse mAb |
| 中文名稱 | |
| 產(chǎn)品類型 | 乙酰化抗體 |
| 抗體來源 | Mouse |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | F7H9 |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-1000,IHC-P=1:50-100,IHC-F=1:50-100,IF=1:50-100,ICC/IF=1:50-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 組蛋白翻譯后修飾（PTMs）是表觀遺傳學(xué)調(diào)控染色質(zhì)結(jié)構(gòu)的關(guān)鍵機(jī)制，被稱為“組蛋白密碼”。組蛋白上的PTMs包括乙酰化、甲基化、磷酸化和新的酰化直接影響染色質(zhì)對(duì)轉(zhuǎn)錄因子的可及性和其他表觀遺傳調(diào)控，改變基因組的穩(wěn)定性，基因轉(zhuǎn)錄等。組蛋白乙酰化主要發(fā)生在核心組蛋白氨基末端的多個(gè)賴氨酸殘基上，以響應(yīng)各種刺激，在基因表達(dá)調(diào)控、DNA損傷修復(fù)、染色質(zhì)動(dòng)力學(xué)等方面發(fā)揮重要作用。大多數(shù)情況下，組蛋白H2A主要在Lys5、9、15和36位處發(fā)生乙酰化修飾。 H2B主要在Lys5、12、15、16和20位處發(fā)生乙酰化修飾。組蛋白H3主要在Lys4、9、14、18、23、27、56和79位處發(fā)生乙酰化修飾。組蛋白H4主要在Lys5、8、12、16和20位處發(fā)生乙酰化修飾。組蛋白乙酰轉(zhuǎn)移酶（HATs）和組蛋白去乙酰化酶（HDACs）是主要的調(diào)節(jié)因子。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: HeLa, N2a, BRL, Mouse spleen, Mouse kidney
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 11 kDa
Observed MW: 11 kDa
Tissue: Mouse kidney
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:200
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60064M
Tissue: Human lung squamous carcinoma
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:200
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60064M
|
