| 產品編號 | bsm-60207R |
| 英文名稱 | Crotonyl-Histone H2B (Lys11) Recombinant Rabbit mAb |
| 中文名稱 | |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | A10A2 |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-1:2000,IHC-P=1:100-500,IHC-F=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 | 組蛋白經過各種酶催化的修飾，包括乙酰化，甲基化，磷酸化，泛素化等。賴氨酸巴豆酰化是新發現的一種控制染色體結構和基因轉錄的可逆修飾。賴氨酸巴豆酰化在從蠕蟲到人的真核細胞中高度保守。組蛋白賴氨酸巴豆酰化獨特的結構和基因組定位表明，它在機制和功能上不同于組蛋白賴氨酸乙酰化。具體地說，在人類體細胞和兔雄性生殖細胞基因組中，組蛋白巴豆酰化標志著活性啟動子或潛在增強子。組蛋白H2B在Lys11位點的巴豆酰化可能在表觀遺傳調控中發揮重要作用，包括染色質重塑和DNA轉錄調控等。 |
| 產品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: HeLa, 2: NIH-3T3, 3: BRL, 4: Rat kidney, 5: Mouse kidney
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 14 kDa
Observed MW: 14 kDa
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+ crotonic acid (100 ng/ml, 18hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 14 kDa
Observed MW: 14 kDa
Tissue: Human breast
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60207R
Tissue: Mouse small intestine
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60207R
Tissue: Rat liver
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60207R
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
