| 產品編號 | bsm-60696R |
| 英文名稱 | ATG9A Recombinant Rabbit mAb |
| 中文名稱 | 自噬相關蛋白9A重組兔單抗 |
| 別 名 | ATG9A_HUMAN; Autophagy-related protein 9A; APG9-like 1; mATG9; APG9L1; MGD3208; |
| 研究領域 | 腫瘤 細胞生物 信號轉導 腫瘤細胞生物標志物 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | R4D8 |
| 交叉反應 | Human |
| 產品應用 | WB=1:500-1000,IHC-P=1:100-500,IHC-F=1:50-100,IF=1:50-100,ICC/IF=1:50-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 94 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ATG9A |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Acts upstream of or within autophagosome assembly. Located in endosome; phagophore assembly site; and trans-Golgi network. [provided by Alliance of Genome Resources, Apr 2022] Subcellular Location: Cytoplasmic vesicle > autophagosome membrane. Golgi apparatus > trans-Golgi network membrane. Late endosome membrane. SWISS: Q7Z3C6 Gene ID: 79065 Database links: Entrez Gene: 79065 Human SwissProt: Q7Z3C6 Human |
| 產品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at
room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: 1: HepG2, 2: HeLa
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 94 kDa
Observed MW: 100 kDa
Tissue: Human thyroid cancer
Section type: Formalin-fixed & Paraffin
embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal
for bsm-60696R
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
