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CK18 Recombinant Rabbit mAb (bsm-60699R)  
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產(chǎn)品編號 bsm-60699R
英文名稱 CK18 Recombinant Rabbit mAb
中文名稱 細胞角蛋白18重組兔單抗
別    名 K1C18_HUMAN; Keratin, type I cytoskeletal 18; KRT18; CYK18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18(CK-18); Keratin-18(K18); PIG46; Keratin 18;  
研究領(lǐng)域 腫瘤  免疫學(xué)  信號轉(zhuǎn)導(dǎo)  腫瘤細胞生物標(biāo)志物  
抗體來源 Rabbit
克隆類型 Recombinant
克 隆 號 R2G7
交叉反應(yīng) Human
產(chǎn)品應(yīng)用 WB=1:500-1000,IHC-P=1:100-500,IHC-F=1:50-100,IF=1:50-100
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 48 kDa
檢測分子量
細胞定位 細胞核 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CK18 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]


SWISS:
P05783

Gene ID:
3875

Database links:

Entrez Gene: 3875 Human

SwissProt: P05783 Human



產(chǎn)品圖片
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: MOLT-4 (Negative control), 2: HeLa, 3: HeLa+ staurosporine (1 μM, 4 h) Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 48 kDa Observed MW: 48, 22 kDa
Tissue: Human breast Section type: Formalin-fixed & Paraffin embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60699R
Tissue: Human lung adenocarcinoma Section type: Formalin-fixed & Paraffin embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60699R
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