產品編號 | bsm-63127R |
英文名稱 | hIgG4 Recombinant Rabbit mAb |
中文名稱 | hIgG4重組兔單抗 |
別 名 | Ig gamma 4 chain C region; IGHG4; IGG4. |
抗體來源 | Rabbit |
克隆類型 | Recombinant |
克 隆 號 | 1C1 |
交叉反應 | Human |
產品應用 | WB=1:500-1:2000,IHC-P=1:200-1:1000,IHC-F=1:200-1:1000,IF=1:200-1:1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 36 kDa |
檢測分子量 | 50 |
性 狀 | Liquid |
免 疫 原 | A synthesized peptide derived from human Human IgG4: 60-120/396 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產品介紹 |
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens |
產品圖片 |
Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with hIgG4 Monoclonal Antibody, Unconjugated (bsm-63127R) at 1:1000 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with hIgG4 Monoclonal Antibody, Unconjugated (bsm-63127R) at 1:1000 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |