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CDK4 Recombinant Rabbit mAb (bsm-63163R)  
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產品編號 bsm-63163R
英文名稱 CDK4 Recombinant Rabbit mAb
中文名稱 周期素依賴性激酶4重組兔單抗
別    名 Cyclin-dependent kinase 4; Cell division protein kinase 4; PSK-J3; PSKJ3; CDK4; Crk3; CMM 3.  
抗體來源 Rabbit
克隆類型 Recombinant
克 隆 號 8E12
交叉反應 Human,Mouse,Rat
產品應用 WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,ICC/IF=1:50-200,IP=1:20-50
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 34 kDa
檢測分子量 34
性    狀 Liquid
免 疫 原 A synthesized peptide derived from human CDK4: 200-303 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
保存條件 Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase.

SWISS:
P11802

Gene ID:
1019

Cdk4為周期素依賴激酶4,主要參與細胞周期的調控,在細胞分化、有絲分裂中起重要作用。目前主要用于各種腫瘤的研究。
產品圖片
25 ug total protein per lane of various lysates (see on figure) probed with CDK4 monoclonal antibody, unconjugated (bsm-63163R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CDK4 Monoclonal Antibody, Unconjugated(bsm-63163R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CDK4 Monoclonal Antibody, Unconjugated(bsm-63163R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CDK4 Monoclonal Antibody, Unconjugated(bsm-63163R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CDK4 Monoclonal Antibody, Unconjugated(bsm-63163R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CDK4 Monoclonal Antibody, Unconjugated(bsm-63163R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CDK4 Monoclonal Antibody, Unconjugated(bsm-63163R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CDK4 Monoclonal Antibody, Unconjugated(bsm-63163R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CDK4 Monoclonal Antibody, Unconjugated(bsm-63163R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CDK4 Monoclonal Antibody, Unconjugated(bsm-63163R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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