| 產(chǎn)品編號 | bsm-49135M |
| 英文名稱 | SARS-CoV Nucleocapsid Mouse mAb |
| 中文名稱 | SARS病毒核衣殼蛋白單克隆抗體 |
| 別 名 | N [SARS coronavirus ShanghaiQXC2]; |
| 研究領(lǐng)域 | 細菌及病毒 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 7C2B |
| 交叉反應 | (predicted: SARS) |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 46 kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | ≥1mg/ml |
| 免 疫 原 | Recombinant SARS N (nucleocapsid): 1-422/422aa |
| 亞 型 | IgG2b |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M PBS (pH7.4) with 0.02% Proclin300. |
| 保存條件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antib |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The nucleocapsid (N) protein of SARS-coronavirus (SARS-CoV) is the key protein for the formation of the helical nucleocapsid during virion assembly. The nucleocapsid (N) protein of SARS-CoV enters the host cell together with the viral RNA and interferes with several cellular processes. Some of these processes involve interactions between SARS-CoV N protein and host-cell proteins. It has also been demonstrated that the SARS-CoV N protein can bind to DNA in vitro. These interactions might have a role in the pathology of SARS. The N protein may be of potential value in vaccine development for specific prophylaxis and treatment against SARS. |
| 產(chǎn)品圖片 |
Sample:
Lane 1: 2019-nCoV Nucleocapsid Protein full length (His tag) Overexpression E.coli Lysate (Cat#: bs-41408P) at 30 ug
Lane 2: Recombinant SARS N (1-422) protein (Cat#: bs-49002P) at 5 ug
Primary: Anti-SARS N (nucleocapsid) (bsm-49135M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 46 kD
Sample:
Lane 1: SARS-CoV-2 N Protein (Cat#: NP) at 4 ug
Lane 2: Recombinant SARS N (1-422) protein (Cat#: bs-49002P) at 5 ug
Lane 3: MERS N protein (Cat#: bs-40068-V08B) at 2 ug
Primary: Anti-SARS N (nucleocapsid) (bsm-49135M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 46 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
