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[KO驗(yàn)證抗體] TAGLN2 Rabbit pAb (bs-55211R)  
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50ul/1180.00元
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產(chǎn)品編號 bs-55211R
英文名稱 [KO驗(yàn)證抗體] TAGLN2 Rabbit pAb
中文名稱 細(xì)胞骨架相關(guān)蛋白2抗體
別    名 CDABP0035; epididymis tissue protein Li 7e; HA1756; KIAA0120; SM22-alpha homolog; TAGL2_HUMAN; Tagln2; Transgelin 2; Transgelin-2.  
研究領(lǐng)域 腫瘤  細(xì)胞生物  腫瘤細(xì)胞生物標(biāo)志物  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應(yīng) Human,Mouse
產(chǎn)品應(yīng)用 WB=1:500-2000,ICC/IF=1:100
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 22 kDa
檢測分子量
細(xì)胞定位 細(xì)胞漿 細(xì)胞外基質(zhì) 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 Recombinant human TAGLN2: 1-199/199 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antib
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The protein encoded by this gene is similar to the protein transgelin, which is one of the earliest markers of differentiated smooth muscle. The specific function of this protein has not yet been determined, although it is thought to be a tumor suppressor. Multiple transcript variants encoding different isoforms have been found for this gene.

Similarity:
Belongs to the calponin family.
Contains 1 calponin-like repeat.
Contains 1 CH (calponin-homology) domain.

SWISS:
P37802

Gene ID:
8407

Database links:

Entrez Gene: 8407 Human

Omim: 604634 Human

SwissProt: P37802 Human

Unigene: 517168 Human



產(chǎn)品圖片
Sample: Lane 1: 293T (Human) Cell Lysate at 25 ug Lane 2: TAGLN2 knockout (KO) 293T (Human) Cell Lysate at 25 ug Primary: Anti-TAGLN2 (bs-55211R) at 1/1000 dilution Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution Predicted band size: 18 kD Observed band size: 20 kD
Sample: Lane 1: Lung (Mouse) Lysate at 40 ug Lane 2: Placenta (Mouse) Lysate at 40 ug Lane 3: Uterus (Mouse) Lysate at 40 ug Lane 4: U251 (Human) Cell Lysate at 30 ug Lane 5: A431 (Human) Cell Lysate at 30 ug Lane 6: U87MG (Human) Cell Lysate at 30 ug Lane 7: U2os (Human) Cell Lysate at 30 ug Lane 8: Huvec (Human) Cell Lysate at 30 ug Lane 9: A673 (Human) Cell Lysate at 30 ug Primary: Anti-TAGLN2 (bs-55211R) at 1/1000 dilution Anti-beta-Actin (bs-0061R) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 22 kD
MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (KO Validated)TAGLN2 polyclonal Antibody, Unconjugated (bs-55211R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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