| 產品編號 | bs-46009P |
| 英文名稱 | Recombinant SARS-Cov-2 Spike RBD protein, C-mFc (HEK293) |
| 中文名稱 | 重組SARS冠狀病毒2 Spike RBD蛋白 |
| 別 名 | coronavirus spike Protein, 2019-nCoV; cov spike Protein, 2019-nCoV; novel coronavirus RBD Protein, 2019-nCoV; novel coronavirus s1 Protein, 2019-nCoV; novel coronavirus spike Protein, 2019-nCoV; RBD Protein, 2019-nCoV; S1 Protein, 2019-nCoV; pike RBD Prot 新冠病毒S蛋白RBD; 新冠S蛋白RBD; SARS-CoV-2 S蛋白RBD; |
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Specific References (1) | bs-46009P has been referenced in 1 publications.
[IF=6.784] Curreli F et al. Stapled Peptides Based on Human Angiotensin-Converting Enzyme 2 (ACE2) Potently Inhibit SARS-CoV-2 Infection In VitromBio.2020 Dec 11;11(6):e02451-20. other ;
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| 理論分子量 | 14.3 kDa |
| 檢測分子量 | 65 |
| 性 狀 | Lyophilized |
| 濃 度 | ＞0.5 mg/ml |
| 物 種 | SARS-Cov-2 |
| 序 列 | 319-532/1273 |
| 純 度 | >95% as determined by Tris-Bis PAGE; >95% as determined by SEC-HPLC |
| 純化方法 | AC |
| 內毒素 | <1.0 EU/μg as determined by LAL |
| 表達系統 | HEK293 cell |
| 活性 | Not tested |
| 標簽 | C-mFc |
| 緩 沖 液 | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| 保存條件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品介紹 |
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. The spike is essential for both host specificity and viral infectivity. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate. |
| 產品圖片 |
The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
