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Rabbit Anti-Spastin/FITC Conjugated antibody (bs-1770R-FITC)
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說 明 書: 50ug  100ug
50ug/1680.00元
100ug/2980.00元
大包裝/詢價

產品編號 bs-1770R-FITC
英文名稱 Rabbit Anti-Spastin/FITC Conjugated antibody
中文名稱 FITC標記的纖維母細胞表面蛋白/Fibroblast Surface Protein抗體
別    名 Spastin; FSP; Fibroblast surface protein; ADPSP; FSP 2; FSP2; KIAA1083; Spast; Spastic paraplegia 4; SPG 4; SPG4; SPAST_HUMAN.  
規格價格 50ug/1680元 購買    100ug/2980元 購買    大包裝/詢價
說 明 書 50ug  100ug
研究領域 免疫學  生長因子和激素  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Mouse, Rat,  (predicted: Human, Chicken, Dog, Pig, Cow, Horse, Rabbit, )
產品應用 Flow-Cyt=1ug/Test IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 68kDa
細胞定位 細胞膜 
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human FSP
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產品介紹 background:
This gene encodes a member of the AAA (ATPases associated with a variety of cellular activities) protein family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. The encoded ATPase may be involved in the assembly or function of nuclear protein complexes. Two transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described but their full length sequences have not been determined. Mutations associated with this gene cause the most frequent form of autosomal dominant spastic paraplegia 4. [provided by RefSeq, Jul 2008]

Subunit:
Homohexamer. Binding to ATP stabilizes the homohexameric form. Binds to microtubules at least in part via the alpha-tubulin and beta-tubulin tails. The hexamer may adopt a ring conformation through which microtubules pass prior to being severed. Does not interact strongly with tubulin heterodimers. Interacts (via MIT domain) with CHMP1B; the interaction is direct. Interacts with ATL1, RTN1, SSNA1 and ZFYVE27.

Subcellular Location:
Membrane; Single-pass membrane protein (Potential). Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Endoplasmic reticulum. Endosome. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Localization to the centrosome is independent of microtubules. Localizes to the midbody of dividing cells, and this requires CHMP1B. Enriched in the distal axons and branches of postmitotic neurons. Isoform 3 is the main endosomal form.

Tissue Specificity:
Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. The short isoforms may predominate in brain and spinal cord.

Similarity:
Belongs to the AAA ATPase family. Spastin subfamily.
Contains 1 MIT domain.

Database links:

Entrez Gene: 6683 Human

Entrez Gene: 50850 Mouse

Entrez Gene: 362700 Rat

Omim: 604277 Human

SwissProt: Q9UBP0 Human

SwissProt: Q9QYY8 Mouse

SwissProt: B2RYN7 Rat

Unigene: 468091 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產品圖片
Blank control : Mouse spleen. Primary Antibody (green line): Rabbit Anti-Spastin/FITC Conjugated antibody (bs-1770R-FITC) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-FITC . Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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