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Rabbit Anti-Sidekick 1/Cy5.5 Conjugated antibody (bs-11164R-Cy5.5)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價

產品編號 bs-11164R-Cy5.5
英文名稱 Rabbit Anti-Sidekick 1/Cy5.5 Conjugated antibody
中文名稱 Cy5.5標記的細胞粘附分子伴侶蛋白1抗體
別    名 FLJ31425; Protein sidekick-1; sdk1; SDK1_HUMAN; sidekick 1 antibody Sidekick homolog 1, cell adhesion molecule (chicken); sidekick1.  
規格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
研究領域 細胞生物  發育生物學  神經生物學  細胞粘附分子  細胞膜蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,  (predicted: Mouse, Rat, Pig, Horse, Sheep, )
產品應用 ICC=1:50-200 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 242kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Sidekick 1
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產品介紹 background:
Cell adhesion molecules influence cell growth, differentiation, embryogenesis, immune response and cancer metastasis by networking information from the extracellular matrix to the cell. Sidekick-1 (SDK1) is a 2,213 amino acid single-pass membrane protein that functions as a cell adhesion molecule by guiding axonal terminals to specific synapses in developing neurons. Existing as three alternatively spliced isoforms, Sidekick-1 is expressed in retinal neurons and contains thirteen fibronectin type-III domains and six Ig-like C2-type (immunoglobulin-like) domains. Sidekick-1 expression is upregulated in glomeruli of patients with HIV-associated nephropathy, where it leads to podocyte dysfunction. The gene encoding Sidekick-1 maps to human chromosome 7p22.2 and murine chromosome 5 G2.

Function:
Cell adhesion protein that guides axonal terminals to specific synapses in developing neurons. Dysregulation of this protein may play an important role in podocyte dysfunction in HIV-associated nephropathy.

Subunit:
Contains 13 fibronectin type-III domains. Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

Subcellular Location:
Membrane.

Tissue Specificity:
Up-regulated in glomeruli in HIV-associated nephropathy. In diseased glomeruli, significantly overexpressed and the expression is no longer restricted to mesangial cells but includes podocytes and parietal epithelial cells.

Similarity:
Contains 13 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

Database links:
UniProtKB/Swiss-Prot: Q7Z5N4.3

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產品圖片
Blank control: U2OS. Primary Antibody (green line): Rabbit Anti-SDK1 antibody (bs-11164R-CY5.5) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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