| 產品編號 | bs-8609R-BF488 |
| 英文名稱 | Rabbit Anti-LGR6/BF488 Conjugated antibody |
| 中文名稱 | BF488標記的G蛋白偶聯受體6抗體 |
| 別 名 | GPCR LGR6; FLJ14471; Gonadotropin receptor; GPCR; Leucine-rich repeat-containing G protein-coupled receptor 6; Leucine-rich repeat-containing G-protein coupled receptor 6; Lgr6; LGR6_HUMAN; VTS20631. |
| 規格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 細胞生物 信號轉導 G蛋白偶聯受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, (predicted: Rat, Rabbit, ) |
| 產品應用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 104kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LGR6 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: G protein-coupled receptors (GPCRs), also designated seven transmembrane (7TM) receptors and heptahelical receptors, are a protein family which interact with G proteins (heterotrimeric GTPases) to synthesize intracellular second messengers such as diacylglycerol, cyclic AMP, inositol phosphates and calcium ions. Their diverse biological functions range from vision and olfaction to neuronal and endocrine signaling and are involved in many pathological conditions. The GPCR family represents the largest class of targets for modern drugs. Leucine-rich repeat-containing G protein-coupled receptor 6 (LGR6) is an orphan-A GPCR with an N-terminal ectodomain comprising variable leucine-rich repeats, a 7TM region and a unique C-terminal intracellular tail. The sequence of LGR6 is similar to TSHR, FSHR and LHR. LGR6 is predominantly expres-sed in the adrenal gland, ovary and uterus. Function: Orphan receptor. Subcellular Location: Cell membrane. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the G-protein coupled receptor 1 family. Contains 15 LRR (leucine-rich) repeats. Contains 1 LRRNT domain. Database links: Entrez Gene: 59352 Human Entrez Gene: 329252 Mouse Omim: 606653 Human SwissProt: Q9HBX8 Human SwissProt: Q3UVD5 Mouse Unigene: 497402 Human Unigene: 279594 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品圖片 |
Blank control: Mouse kidney.
Primary Antibody (green line): Rabbit Anti-LGR6 antibody (bs-8609R-BF488)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
