| 產品編號 | bs-2327R-AP |
| 英文名稱 | Rabbit Anti-Hepatitis B Virus Surface Antigen/AP Conjugated antibody |
| 中文名稱 | 堿性磷酸酶（AP）標記的人乙肝病毒pre S1/S2蛋白抗體 |
| 別 名 | Hepatitis B Virus Surface Antigen; Hepatitis B virus S1/S2 protein; Hepatitis B virus PreS1; Hepatitis B virus pre S1 protein; HBV pre S1 protein; HBSAG_HBVD2; HBSAG_HBVC5; Hepatitis B Virus Major surface antigen; Large envelope protein; Large surface protein; L glycoprotein; Major surface antigen; Large S protein; L-HBsAg; LHB. |
| 規格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 細胞生物 免疫學 細菌及病毒 細胞表面分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | |
| 產品應用 | WB=1:50-200 IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 31/44kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from HBSAG_HBVD2 pre S1/S2 protein |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr. Function: Li-HBsAg interacts with capsid protein and with HDV Large delta antigen. Isoform M associates with host chaperone CANX through its pre-S2 N glycan. This association may be essential for M proper secretion. Subunit: Li-HBsAg interacts with capsid protein and with HDV Large delta antigen. Isoform M associates with host chaperone CANX through its pre-S2 N glycan. This association may be essential for M proper secretion. Subcellular Location: Virion membrane. Post-translational modifications: Isoform M is N-terminaly acetylated at a ratio of 90%, and N-glycosylated at the pre-S2 region. Myristoylated. Similarity: Belongs to the orthohepadnavirus major surface antigen family. Database links: Entrez Gene: 2703546 Hepatitis B virus SwissProt: P03142 Hepatitis B virus Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 乙型肝肝炎病毒顆粒直徑42nm，由包膜與核衣殼組成，包膜含有碳水化合物、蛋白質和脂類。其中蛋白質為主要抗原成分，包括S抗原：pre-s1區和pre-s2區，這些抗原成分在病毒侵入細胞過程中有著至關重要的作用，為深入研究乙型肝肝炎病毒發病機理及變異有著至關重要的作用。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
