| 產(chǎn)品編號 | bs-4529R-BF350 |
| 英文名稱 | Rabbit Anti-NDV HN protein/BF350 Conjugated antibody |
| 中文名稱 | BF350標記的雞新城疫血凝素－神經(jīng)氨酸酶抗體 |
| 別 名 | hemagglutinin-neuraminidase protein; newcastle disease virus(NDV); HN protein; hemagglutinin-neuraminidase protein [Newcastle disease virus]; HN_NDVB; Newcastle disease virus HN protein; Newcastle disease virus hemagglutinin neuraminidase protein. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 免疫學(xué) 細菌及病毒 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | |
| 產(chǎn)品應(yīng)用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 63kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from NDV HN protein |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: The entry of Newcastle disease virus (NDV), a prototype paramyxovirus, is directed by two virion glycoproteins, the hemagglutinin-neuraminidase (HN) protein and the fusion (F) protein . HN protein, the virus attachment protein, binds to sialic acid-containing receptors, and F protein mediates membrane fusion. In contrast to many viral fusion proteins, paramyxovirus F proteins do not require the acid pH of endosomes to activate fusion activity. As a consequence, infected cells expressing both attachment proteins and F proteins can fuse with adjacent cells to form multinuclear cells, or syncytia, a process that is assumed to mimic virus-cell fusion . Function: Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion (By similarity). Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins (By similarity). Subcellular Location: Virion membrane; Single-pass type II membrane protein (Potential). Host cell membrane; Single-pass type II membrane protein (Potential). Similarity: Belongs to the paramyxoviruses hemagglutinin-neuraminidase family. Database links: Entrez Gene: 912270 NDV Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
