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Rabbit Anti-phospho-MLF1 Interacting Protein(Thr78)/FITC Conjugated antibody (bs-5753R-FITC)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價
產品編號 bs-5753R-FITC
英文名稱 Rabbit Anti-phospho-MLF1 Interacting Protein(Thr78)/FITC Conjugated antibody
中文名稱 FITC標記的磷酸化卡波西氏肉瘤皰疹病毒潛伏核抗原相互作用蛋白1抗體
別    名 MLF1 Interacting Protein/PBIP1(phospho T78); MLF1 Interacting Protein/PBIP1 (phospho T78);MLF1 Interacting Protein (phospho T78); MLF1 Interacting Protein (phospho Thr78); p-MLF1 Interacting Protein (Thr78); CENP U; CENP50; CENPU; centromere protein U; HHV8 LNAIP1; ICEN24; Kaposi Sarcoma Herpesvirus latent nuclear antigen interacting protein 1; KLIP-1; KLIP1; KSHV latent nuclear antigen interacting protein 1; KSHV LNAIP1; LNAIP 1; LNAIP-1; MLF1IP1; PBIP1; CENPU_HUMAN.  
規格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
產品類型 磷酸化抗體 
研究領域 腫瘤  免疫學  信號轉導  細胞周期蛋白  激酶和磷酸酶  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 (predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep, )
產品應用 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 48kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthesised phosphopeptide derived from human MLF1 Interacting Protein around the phosphorylation site of Thr78
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產品介紹 background:
MLF1 Interacting protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a component of the CENPA nucleosome-associated complex (CENPA NAC), which plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. This protein is involved in transcriptional repression and interacts with the N-terminal domain of Kaposi Sarcoma Herpesvirus latent nuclear antigen (KSHV LNA).

Function:
Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression.

Subunit:
Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and MLF1IP/CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. Interacts with the N-terminal domain of Kaposi's sarcoma-associated herpesvirus latent nuclear antigen (LNA). Interacts with MLF1. Interacts with PLK1.

Subcellular Location:
Cytoplasm. Nucleus. Chromosome, centromere, kinetochore. Note=Localizes in the kinetochore domain of centromeres. Colocalizes with PLK1 at the interzone between the inner and the outer kinetochore plates.

Tissue Specificity:
Expressed at high levels in the testis, fetal liver, thymus, bone marrow and at lower levels in the lymph nodes, placenta, colon and spleen. Present in all cell lines examined, including B-cells, T-cells, epithelial cells and fibroblast cells. Expressed at high levels in glioblastoma cell lines.

Post-translational modifications:
Phosphorylated by PLK1 at Thr-78, creating a self-tethering site that specifically interacts with the polo-box domain of PLK1.

Database links:

Entrez Gene: 617866 Cow

Entrez Gene: 79682 Human

Entrez Gene: 71876 Mouse

Entrez Gene: 306464 Rat

Omim: 611511 Human

SwissProt: Q2KIW5 Cow

SwissProt: Q71F23 Human

SwissProt: Q8C4M7 Mouse

SwissProt: Q4V8G7 Rat

Unigene: 575032 Human

Unigene: 217385 Mouse

Unigene: 22108 Mouse

Unigene: 128609 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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