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Rabbit Anti-phospho-DDX5 (Tyr593)/BF350 Conjugated antibody (bs-8005R-BF350)
訂購熱線:400-901-9800
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訂購QQ:  400-901-9800
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價
產品編號 bs-8005R-BF350
英文名稱 Rabbit Anti-phospho-DDX5 (Tyr593)/BF350 Conjugated antibody
中文名稱 BF350標記的磷酸化三磷酸腺苷依賴解旋酶ddx5抗體
別    名 DDX5 (phospho Y593); DDX5 (phospho Tyr593); p-DDX5 (Y593); p-DDX5 (Tyr593); ATP dependent RNA helicase DDX5; DDX 5; Ddx5; DDX5_HUMAN; DEAD (Asp Glu Ala Asp) box helicase 5; DEAD (Asp Glu Ala Asp) box polypeptide 5; DEAD box 5; DEAD box protein 5; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5 (RNA helicase, 68kD); DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase 68kDa); DEAD/H box-5 (RNA helicase 68kD); DKFZp686J01190; G17P1; HELR; HLR 1; HLR1; HUMP68; P68; P68; p68 RNA helicase; Probable ATP dependent RNA helicase DDX5; Probable ATP-dependent RNA helicase DDX5; RNA helicase 68kD; RNA helicase p68; RNA-dependent ATPase.  
規格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
產品類型 磷酸化抗體 
研究領域 發育生物學  神經生物學  信號轉導  激酶和磷酸酶  細胞分化  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, Rat,  (predicted: Mouse, Cow, Horse, Rabbit, )
產品應用 ICC=1:50-200 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 69kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthesised phosphopeptide derived from human DDX5 around the phosphorylation site of Tyr593
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產品介紹 background:
p68 RNA helicase is a nuclear protein that exhibits RNA-dependent ATPase activity. Phosphorylation by protein kinase C inhibits p68 RNA helicase activity. p68 RNA helicase appears to play a role in organ differentiation during development. Furthermore, p68 RNA helicase is expressed in early neural development and in various mesodermal tissues in a number of different chordate embryos. At the cellular level, the expression levels of p68 RNA helicase increases in serum-induced quiescent cell lines. p68 RNA helicase may function as a coactivator for estrogen receptor alpha. Additionally, p68 RNA helicase associates with transcriptional coactivators CBP and p300. p68 RNA helicase localizes to the nucleus under normal conditions. During late telophase, p68 RNA helicase and fibrillarin colocalize to nascent nucleoli. p68 RNA helicase may function as a heterodimer with p72 RNA helicase.

Function:
RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing.

Subunit:
Interacts with BRDT (By similarity). Identified in thespliceosome C complex. Interacts with RBM4; the interaction occursin a RNA-independent manner. Interacts with EIF2C1 and EIF2C2.Interacts with ESR1; the interaction is enhanced by phosphorylationof ESR1 AF-1 domain. Interacts with AR, NCOA1, NCOA2, NCOA3, EP300,CREBBP, POLR2A, TP53, RUNX2 and HDAC1. Self-associates. Interactswith DDX17. [INTERACTION] Q12873:CHD3; NbExp=4; IntAct=EBI-351962, EBI-523590;P45481:Crebbp (xeno); NbExp=3; IntAct=EBI-351962, EBI-296306;Q09472:EP300; NbExp=4; IntAct=EBI-351962, EBI-447295; P03372:ESR1;NbExp=8; IntAct=EBI-351962, EBI-78473; P22087:FBL; NbExp=6;IntAct=EBI-351962, EBI-358318; Q13547:HDAC1; NbExp=4;IntAct=EBI-351962, EBI-301834; O95983:MBD3; NbExp=4;IntAct=EBI-351962, EBI-1783068; P10085:Myod1 (xeno); NbExp=3;IntAct=EBI-351962, EBI-4405734; P24928:POLR2A; NbExp=3;IntAct=EBI-351962, EBI-295301; Q08775-3:Runx2 (xeno); NbExp=2;IntAct=EBI-351962, EBI-6119991; P04637:TP53; NbExp=3;IntAct=EBI-351962, EBI-366083; P04637-1:TP53; NbExp=2;IntAct=EBI-351962, EBI-3895849; P04637-7:TP53; NbExp=2;IntAct=EBI-351962, EBI-3895873.

Subcellular Location:
Nucleus.

Post-translational modifications:
Arg-502 is dimethylated, probably to asymmetricdimethylarginine.
Sumoylated; sumoylation, promoted by PIAS1, promotesinteraction with HDAC1 and transcriptional repression activity.Sumoylation also significantly increases stability, and reducespolyubiquitination.
Polyubiquitinated, leading to proteasomal degradation.

Similarity:
Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.

Database links:

Entrez Gene: 1655 Human

Entrez Gene: 13207 Mouse

Entrez Gene: 287765 Rat

Omim: 180630 Human

SwissProt: P17844 Human

SwissProt: Q61656 Mouse

Unigene: 279806 Human

Unigene: 220038 Mouse

Unigene: 162208 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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