| 產(chǎn)品編號 | bs-13496R-BF647 |
| 英文名稱 | Rabbit Anti-GPAA1/BF647 Conjugated antibody |
| 中文名稱 | BF647標記的糖基磷脂酰肌醇錨連接蛋白1抗體 |
| 別 名 | Anchor attachment protein 1 (Gaa1p, yeast) homolog; GAA1; GAA1; GAA1; GAA1 protein homolog; Glycophosphatidylinositol anchor attachment 1; Glycosylphosphatidylinositol anchor attachment 1 protein; Glycosylphosphatidylinositol anchor attachment 1 protein; Glycosylphosphatidylinositol anchor attachment 1 protein; Glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast); GPAA 1; GPAA1; GPAA1_HUMAN; GPAA1P anchor attachment protein 1 homolog; GPI anchor attachment protein 1; GPI anchor attachment protein 1; GPI anchor attachment protein 1; GPI transamidase subunit; hGAA1. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 細胞生物 細胞表面分子 細胞類型標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human, Mouse, Rat, Pig, Cow, Rabbit, Sheep, ) |
| 產(chǎn)品應(yīng)用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 68kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPAA1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Glycosylphosphatidylinositol (GPI) acts as a membrane anchor for cell surface proteins. Glycosylphosphatidylinositol anchor attachment 1 protein (GPAA1), also designated GPI anchor attachment protein 1 or GAA1 protein homolog, is a membrane protein localized to the endoplasmic reticulum which is involved in GPI-anchor biosynthesis. GPAA1 is crucial for GPI-anchoring of precursor proteins and catalyzes the attachment of GPI to proteins containing a C-terminal GPR attachment signal. GAA1 contains an N-terminal signal sequence, one cAMP- and cGMP-dependent protein kinase phosphorylation site, two potential N-glycosylation sites, one leucine zipper pattern and eight putative transmembrane domains. GPAA1 is ubiquitously expressed and shows higher levels of expression in fetal tissues than in adult tissues. Function: Essential for GPI-anchoring of precursor proteins but not for GPI synthesis. Acts before or during formation of the carbonyl intermediate. Subunit: Forms a complex with PIGK/GPI8, PIGT, PIGU and PIGS. Subcellular Location: Endoplasmic reticulum membrane. Tissue Specificity: Ubiquitously expressed in fetal and adult tissues. Expressed at higher levels in fetal tissues than adult tissues. Database links: UniProtKB/Swiss-Prot: O43292.3 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
