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Rabbit Anti-CD14/BF647 Conjugated antibody (bs-20796R-BF647)
訂購熱線:400-901-9800
訂購郵箱:sales@bioss.com.cn
訂購QQ:  400-901-9800
技術支持:techsupport@bioss.com.cn
說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價

產品編號 bs-20796R-BF647
英文名稱 Rabbit Anti-CD14/BF647 Conjugated antibody
中文名稱 BF647標記的內毒素受體抗體
別    名 CD14 antigen; CD14 molecule; Lipopolysaccharide receptor; LPSR; Monocyte Differentiation Antigen 14; Monocyte differentiation antigen CD14; Myeloid cell specific leucine rich glycoprotein; CD14_HUMAN; Myeloid cell-specific leucine-rich glycoprotein.  
規格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
研究領域 轉錄調節因子  細胞膜受體  糖尿病  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, Mouse, 
產品應用 Flow-Cyt=2ug/Test ICC=1:50-200 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 35/40kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CD14
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產品介紹 background:
CD14 is a single copy gene encoding 2 protein forms: a 50 to 55 kDa glycosylphosphatidylinositol anchored membrane protein (mCD14) and a monocyte or liver derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS) dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram negative shock. CD14 also appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.

Function:
Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

Subunit:
Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, MD-2 and TLR4.

Subcellular Location:
Cell membrane; Lipid-anchor, GPI-anchor.

Similarity:
Contains 11 LRR (leucine-rich) repeats.

Database links:

Entrez Gene: 929 Human

Entrez Gene: 12475 Mouse

Omim: 158120 Human

SwissProt: P08571 Human

Unigene: 163867 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

CD14又稱內毒素受體,屬細胞表面糖蛋白家族成員之一。在機體免疫、防御系統引起的一系列反應中介導細胞識別LPS并引起細胞酪氨酸磷酸化、核因子NF-κB轉位、觸發細胞因子釋放和氧自由基產生。CD14在細胞因子產生的調控方面起重要作用,因而對于CD14結構、功能及其臨床意義的研究日益受到重視。
產品圖片
Blank control:HL-60. Primary Antibody (green line): Rabbit Anti-CD14 antibody (bs-20796R-BF647) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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