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Rabbit Anti-CSFV E2/HRP Conjugated antibody (bs-20116R-HRP)
訂購熱線:400-901-9800
訂購郵箱:sales@bioss.com.cn
訂購QQ:  400-901-9800
技術支持:techsupport@bioss.com.cn
說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價
產品編號 bs-20116R-HRP
英文名稱 Rabbit Anti-CSFV E2/HRP Conjugated antibody
中文名稱 辣根過氧化物酶標記的豬瘟病毒E2抗體
別    名 CSFV Polyprotein; CSFV E2 protein; CSFV E2; polyprotein [Classical swine fever virus]; polyprotein; CSFV polyprotein; E2; Envelope glycoprotein E2; CSFV Envelope glycoprotein E2; Classical swine fever virus E2; Classical swine fever virus Envelope glycoprotein E2; structural protein E2 [Classical swine fever virus].  
規格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
研究領域 免疫學  細菌及病毒  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應
產品應用 WB=1:500-2000 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 42kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 CSFV E2 protein
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產品介紹 background:
Classical swine fever(CSF) is a kind of acute and intense contagious disease in swine, it material damages swine industry. CSF could cause swine appearing disseminated hemorrhage in systemic organ and tissue, degeneration and necrosis in small vascular and capillary endothelial cells. Classical swine fever virus (CSFV) belongs to genera Pestivirus, family Flaviviridae, it’s genome total length is 12.3Kb,including a big ORF, encoding 11 structural and nonstructural proteins.

Function:
E(rns), E1 and E2 are responsible of cell attachment and subsequent fusion of viral and cellular membrane.
P7 forms a leader sequence to properly orient NS2 in the membrane.
Uncleaved NS2-3 is required for production of infectious virus.
NS2 protease seems to play a vital role in viral RNA replication control and in the pathogenicity of the virus.
NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase.
NS4A is a cofactor for the NS3 protease activity.
RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome.

Subunit:
The E(rns) glycoprotein is found as a homodimer; disulfide-linked. The E1 and E2 envelope glycoproteins form disulfide-linked homodimers as well as heterodimers.

Subcellular Location:
Host cell surface.

Post-translational modifications:
The E(rns) glycoprotein is heavily glycosylated.
The viral RNA of pestiviruses is expressed as a single polyprotein which undergoes post-translational proteolytic processing resulting in the production of at least eleven individual proteins. The N-terminal protease cleaves itself from the nascent polyprotein autocatalytically and thereby generates the N-terminus of the adjacent viral capsid protein C.
Cleavage between E2 and p7 is partial.

Similarity:
Belongs to the pestivirus polyprotein family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 peptidase C53 domain.
Contains 1 peptidase C74 domain.
Contains 1 peptidase S31 domain.
Contains 1 RdRp catalytic domain.

Database links:

Entrez Gene: 920146 CSFVA

SwissProt: P19712 CSFVA



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

豬瘟(Classical Swine Fever,CSF)是由豬瘟病毒(CSFV)引起的豬急性高度接觸性傳染性疾病,是嚴重威脅養豬業,具有重要經濟意義的病毒性疾病之一,豬瘟可引起豬的全身組織器官彌漫性出血,小血管和毛細血管內皮細胞發生變性、壞死。
豬瘟病毒為黃病毒科(Flaviviridae)瘟病毒屬(Pestivirus)成員,基因組大小約12.3kb,含一個大的開放閱讀框(ORF),由11 個編碼結構蛋白與非結構蛋白的基因組成。豬瘟病毒對血管組織具有親嗜性,病理組織學檢查時發現,組織器官的出血斑點主要是由于小血管和毛細血管內皮細胞發生腫脹、變性和壞死;梗死灶的發生主要是由于小動脈管內皮細胞發生變性、壞死、剝脫后,血管內膜粗糙,官腔內血栓形成,導致官腔狹窄或閉塞所致。此外,還可見到心肌呈實質變性,但病豬的腎臟等器官的實質細胞沒有變性、壞死等病理變化。
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