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Rabbit Anti-CSFV E2/BF488 Conjugated antibody (bs-20116R-BF488)
訂購熱線:400-901-9800
訂購郵箱:sales@bioss.com.cn
訂購QQ:  400-901-9800
技術(shù)支持:techsupport@bioss.com.cn
說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價(jià)
產(chǎn)品編號(hào) bs-20116R-BF488
英文名稱 Rabbit Anti-CSFV E2/BF488 Conjugated antibody
中文名稱 BF488標(biāo)記的豬瘟病毒E2抗體
別    名 CSFV Polyprotein; CSFV E2 protein; CSFV E2; polyprotein [Classical swine fever virus]; polyprotein; CSFV polyprotein; E2; Envelope glycoprotein E2; CSFV Envelope glycoprotein E2; Classical swine fever virus E2; Classical swine fever virus Envelope glycoprotein E2; structural protein E2 [Classical swine fever virus].  
規(guī)格價(jià)格 100ul/2980元 購買        大包裝/詢價(jià)
說 明 書 100ul  
研究領(lǐng)域 免疫學(xué)  細(xì)菌及病毒  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng)
產(chǎn)品應(yīng)用 ICC=1:50-200 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 42kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 CSFV E2 protein
亞    型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
Classical swine fever(CSF) is a kind of acute and intense contagious disease in swine, it material damages swine industry. CSF could cause swine appearing disseminated hemorrhage in systemic organ and tissue, degeneration and necrosis in small vascular and capillary endothelial cells. Classical swine fever virus (CSFV) belongs to genera Pestivirus, family Flaviviridae, it’s genome total length is 12.3Kb,including a big ORF, encoding 11 structural and nonstructural proteins.

Function:
E(rns), E1 and E2 are responsible of cell attachment and subsequent fusion of viral and cellular membrane.
P7 forms a leader sequence to properly orient NS2 in the membrane.
Uncleaved NS2-3 is required for production of infectious virus.
NS2 protease seems to play a vital role in viral RNA replication control and in the pathogenicity of the virus.
NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase.
NS4A is a cofactor for the NS3 protease activity.
RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome.

Subunit:
The E(rns) glycoprotein is found as a homodimer; disulfide-linked. The E1 and E2 envelope glycoproteins form disulfide-linked homodimers as well as heterodimers.

Subcellular Location:
Host cell surface.

Post-translational modifications:
The E(rns) glycoprotein is heavily glycosylated.
The viral RNA of pestiviruses is expressed as a single polyprotein which undergoes post-translational proteolytic processing resulting in the production of at least eleven individual proteins. The N-terminal protease cleaves itself from the nascent polyprotein autocatalytically and thereby generates the N-terminus of the adjacent viral capsid protein C.
Cleavage between E2 and p7 is partial.

Similarity:
Belongs to the pestivirus polyprotein family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 peptidase C53 domain.
Contains 1 peptidase C74 domain.
Contains 1 peptidase S31 domain.
Contains 1 RdRp catalytic domain.

Database links:

Entrez Gene: 920146 CSFVA

SwissProt: P19712 CSFVA



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

豬瘟(Classical Swine Fever,CSF)是由豬瘟病毒(CSFV)引起的豬急性高度接觸性傳染性疾病,是嚴(yán)重威脅養(yǎng)豬業(yè),具有重要經(jīng)濟(jì)意義的病毒性疾病之一,豬瘟可引起豬的全身組織器官彌漫性出血,小血管和毛細(xì)血管內(nèi)皮細(xì)胞發(fā)生變性、壞死。
豬瘟病毒為黃病毒科(Flaviviridae)瘟病毒屬(Pestivirus)成員,基因組大小約12.3kb,含一個(gè)大的開放閱讀框(ORF),由11 個(gè)編碼結(jié)構(gòu)蛋白與非結(jié)構(gòu)蛋白的基因組成。豬瘟病毒對血管組織具有親嗜性,病理組織學(xué)檢查時(shí)發(fā)現(xiàn),組織器官的出血斑點(diǎn)主要是由于小血管和毛細(xì)血管內(nèi)皮細(xì)胞發(fā)生腫脹、變性和壞死;梗死灶的發(fā)生主要是由于小動(dòng)脈管內(nèi)皮細(xì)胞發(fā)生變性、壞死、剝脫后,血管內(nèi)膜粗糙,官腔內(nèi)血栓形成,導(dǎo)致官腔狹窄或閉塞所致。此外,還可見到心肌呈實(shí)質(zhì)變性,但病豬的腎臟等器官的實(shí)質(zhì)細(xì)胞沒有變性、壞死等病理變化。
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